Plant Tissue Culture (Theory and Practicals)

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Plant Tissue Culture brings together information on widest range of topics including practicals. Written in a concise manner and ideally suited for students and teachers at colleges and Universities. At the outset History of Plant Tissue culture is given. It is followed by Laboratory requirements, Culture media and methods of sterilization. Subsequent chapters include Cell culture, somatic embryogenesis, synthetic seeds, micropropagation, haploid production, nucellus, ovule, ovary and flower culture, embryo and endosperm culture, protoplast culture and somatic hybridization, somaclonal variations and germplasm conservation. At the end secondary metabolite production and isolation of mutants from cell culture are given. The theory is followed by practicals. Nine experiments usually carried out by students at Post Graduate and Graduate level are given. The book covers the syllabus of M.Sc. Botany, Biotechnoly, B. Tech. Biotechnology, B.Sc. and M.Sc. Agriculture of all the Indian Universities.

1. History of Plant Tissue Culture; 2. Laboratory Requirements for Plant Tissue Culture; 3. Tissue Culture Media; 4. Methods of Sterilization; 5. Cell Culture; 6. Somatic Embryogenesis; 7. Synthetic Seeds; 8. Micropropagation of Higher Plants; 9. Haploid Production; 10. Nucellus Culture, Ovule Culture, Ovary Culture and Flower Culture; 11. Embryo and Endosperm Culture; 12. Protoplast Culture and Somatic Hybridization; 13. Somaclonal Variations; 14. Germplasm Conservation; 15. Production of Secondary Metabolites through Tissue Culture; 16. Isolation of Mutants from Cell Culture