Section I:

HISTOPATHOLOGY

1. Methods of histopathological processing

2. Automated tissue Processor

3. Microtome

4. Grossing technique

5. Use & care of Microscope

6. Decalcification

7. Biomedical waste management

8. Various mounting techniques in histopathology museum

9. Honing & stropping

10. Special stain for Carbohydrate

11. Microtome knives

12. Block making & cutting (Steps of paraffin wax embedding)

13. Tissue floating water bath

14. Different type of fixatives

15. Haematoxylin & Eosin staining

16. Automation in histopathology

17. Dark ground microscopy

18. Organisation of histopathology & the various equipment in histopathology lab

19. Cryo state & Caryo centrifuge

20. FNAC

21. Special stain for collagen fiber

22. ICDS classification & coding

23. Application of computer in pathology

24. Electron microscope

25. Reticulin stain

26. Bouin’s fluid

27. Fluorescent microscope

28. Dehydrating agents

29. Special stain for muscle fiber

30. HPLC & it’s uses

31. Inflammation

32. Types of Microscope

Section II:

CLINICAL PATHOLOGY

1. Collection, preservation, examination of urine

2. Semen analysis

3. Collection/transport/preservation/processing of body fluids

4. Malignant cell examination in different body fluid

5. Sputum examination

6. CSF examination

7. Hemosiderin in urine

8. Lumber puncture needle

Section III:

HAEMATOLOGY

1. PBF staining by Leishman’s staining

2. Red cell indices

3. Granulocytes

4. PCV

5. Various methods of Erythrocyte sedimentation rate estimation

6. APTT (Activated partial thromboplastin time)

7. Methods of Hb estimation with their advantages & disadvantages

8. Screening of sickle cell anemia

9. Qualities of a good peripheral blood smear

10. Various anticoagulants used in blood sample along with their uses

11. Blood sample collection (Phlebotomy)

12. Laboratory safety procedures & First aid in Laboratory accidents

13. Prothrombin time

14. Indications for serum collection

15. Eosinophilia

16. Bleeding time

17. Sickling test

18. Normal blood leucocytes

19. Osmotic fragility test

20. Various instruments and glassware used in hematology

21. Drabkin’s reagent

22. Differentiate between ESR & PCV

23. Differentiate between BT & CT

24. Differentiate between Erythropoiesis & leucopoiesis

25. Differentiate between SI & conventional unit

26. Hemostasis

27. Tests for iron deficiency anemia

28. Polycythemia

29. Test for G6PD deficiency

30. Thrombopoiesis & Various test for bleeding disorder

31. Schilling test

32. Hemolytic anemia

33. Preparation of PBF, morphology and function of cells seen in PBF

34. Platelet count

35. Abnormal red cell

36. Reticulocyte count

37. Hemopoiesis

38. Various types of hemoglobin & Hb electrophoresis

39. Clotting factors & the lab diagnosis of coagulation disorders

40. Megaloblastic anemia

41. Quality control for hematology analyzer

42. Labelled diagram LE cell

43. Sickle cell anemia

44. Hemosiderin

45. Differential diagnosis of microcytic anemia

46. Clot lysis test

47. Parasites seen in PBF

48. Vit B12 assay

49. Iron studies

50. Red cell distribution width

51. Lymphoblast

52. Leukemia

53. Venesection procedure

54. Hazards in clinical laboratory

55. Plasma cell

Section IV:

CYTOLOGY

1. Fixation of cytosmear & stain used, methods of staining, their advantages

2. Stain used in cytology, staining methods, advantages & disadvantages of each method

3. Normal cell in cervical cytology

4. Cell cycle

5. Normal lymph node histology

6. Normal structure of cell

7. Preparation of cell block

8. Types of specimen in cytology

9. FNAC

10. Procedure of cervical cytology & breast lymph FNAC along with their benign & malignant conditions

11. Cytological features of cancer

12. Histology of mammary gland in normal breast

13. Urinary cytology

14. Diagnosis of malignant effusion

15. CSF examination in tubercular meningitis

16. Respiratory Exfoliative cytology, collection, preparation & staining of cervical smears

17. Principle reagents & methods of PAP staining with advantages / disadvantages

18. Histology of thyroid gland

19. Hormonal cytology & cytological indices

20. Shorr’s staining

21. Normal Histology of uterine cervix

22. Cytology of pleural fluids in various disease

23. Liquid base cytology

Section V:

AUTOMATION IN CYTOLOGY

1. Automation in exfoliative cytology

2. Flowcytometry

Section VI:

TISSUE CULTURE & IMMUNOHISTOCHEMISTRY

1. Tissue culture

2. Preservation of immortalised cell lines

3. Laminar airflow

4. Inverted microscope

5. Incubator or CO2

Incubator

Section VII:

CYTOGENETICS

1. Chromosome

2. G-Banding

3. Barr body

4. Karyotyping

5. Oncogene

6. Nucleic acid testing (NAT)

Section VIII:

IMMUNO-CYTO-CHEMISTRY

1. Basic concept of immuno-cyto-chemistry

2. Monoclonal antibodies

Section IX:

IMMUNO-HEMATOLOGY AND BLOOD TRANSFUSION

[BLOOD BANKING]

1. Bombay / Rare blood group

2. Blood transfusion safety precaution

3. Rhesus Factor

4. ABO & Rh Grouping

5. Pre-transfusion testing of blood & cross matching

6. Screening of blood donor

7. Coomb’s test

8. Anticoagulants used in blood banks

9. Transfusion transmitted diseases

10. Antigen-antibody reaction

11. Blood component in blood bank

12. Immunity

13. HLA antigen

14. Autologous blood transfusion

15. Subtypes of blood group A 34