DNA-based analytical techniques have been in use since sometime while DNA barcoding is in vogue from 2003. DNA technology and the research on analytics, ap-plications and paradigms have evolved and diversified it. At the beginning, the application of the technique was limited to animal kingdom; then, it was adapted by plant science researchers. The paper presents the domains where the technique is widely used in plant science. The general ability to use DNA-based markers for identifica-tion and discrimination between different ranks of taxa enabled the spread of its applicability in a variety of subject areas like taxonomy, phylogeny, analytical uses like biodiversity, biosafety, biosecurity, forensic and eco-logical studies at both population and community levels, etc. A brief account of DNA barcoding tools and tech-niques used are presented along with advances in the strategies for DNA barcoding and the limitations.