Two less known sacred groves viz. ‘Dariabaichi Rai’ and ‘Birobachi Rai’ in Otur range of Junnar taluka, Pune in Maharashtra were surveyed botanically. About 200 species were collected/ noted from the area, processed and deposited in the regional herbarium. Out of which c 100 species are used by tribals and local people for their day to day life, e.g. food, shelter, medicines, etc. Relative data of such species viz. Casearia tomentosa, Cocculus hirsutus, Heterophragma quadriloculare, Meyna laxiflora, Paracalyx scariosus, Radermachera xylocarpa, Verbascum coromandelianum, etc, are given along with their correct and local names, families, parts used, etc. Biotic factors affecting the important plant wealth and groves with possible conservation aspects are also discussed.