Panna National Park lies in Vindhyan hill ranges of Madhya Pradesh between 24º 27' to 24º 46' North latitudes and 71º 45' to 80º 90' East longitudes, covering an area of 478.81 sq. km. The present paper deals with the topography, geology, climate, rainfall and soil in general. Floris-tically, the present investigation is primarily based on 3 years extensive tours undertaken in different seasons along with available herbarium data in BSA, BSD and CAL; critical investi-gation of these resulted in to 609 species under 394 genera and 108 families. The vegetation of the area is discussed along with seasonal vegetation, hydophytic vegetation, vegetation of special habitats and weeds of Kharif and Rabi crops. A separate chapter deals with interaction of plants and animals and their favourable habitat and also plant species used as staple food by different animals of Panna National Park. Economic aspects of the flora, viz. cultivated crops, vegetable plants, fruit yielding plants, ornamental plants, wild edible plants, timber yielding plants, fibre yielding plants, tannin and gum yielding plants and medicinal plants have been dealt briefly. Conservational aspect of the flora has also been discussed in brief for the benefit of policy makers, foresters and other plant managers.