Fruit rot of ber causes considerable damage to fruit of ber. The efficacy of eight medicinal and aromatic plant extracts and three bio inoculants was evaluated in vitro and in vivo for their antifungal activity against Alternaria alternata. Extracts of Allium sativum (bulb), Curcuma longa (rhizomes), Azadirachta indica (leaves) and Diopyros cordifolia (fruits) effectively inhibited the growth of pathogen and these extracts were also tested as pre and post inoculation against the pathogen. Bio-control agents i.e. Trichoderma harzianum and T. viride which were also evaluated and could suppress the growth of A. alternata, effectively on PDA medium when tested by dual culture technique. The investigation suggested that fruit rot can be effectively minimized when the fruits were pre inoculated with in an extract of A. sativum @20% (w/v) or Trichoderma harzianum@106 conidal suspension/ml.