Ceropegia bulbosa Roxb. var. bulbosa is a threatened medicinal geophyte native to the arid regions of the Thar Desert, exhibiting unique adaptations such as tuber formation and Crassulacean Acid Metabolism for survival under extreme environmental conditions. Due to overexploitation and habitat degradation, its natural populations are declining, necessitating efficient conservation and propagation strategies. The present study evaluates aeroponic propagation using auxins (IBA, NAA and IAA) at 500 and 1000 μM with 5-and 10-min treatment respectively. Stem cuttings were assessed for rooting, growth and tuber development under controlled conditions. Auxin treatments significantly improved rooting induction compared to control. The fastest root initiation was observed in IBA (1000 μM) (2.47 ± 0.52 days), followed by IBA (500 μM) (2.53 ± 0.52 days) and NAA (1000 μM) (4.33 ± 0.49 days), whereas control required 9.27 ± 0.46 days. At one month, IBA (500 μM) showed maximum root length (6.13 ± 1.27 cm), followed by NAA (5.99 ± 1.09 cm) and IAA (4.96 ± 1.39 cm), while control showed limited elongation (2.61 ± 0.66 cm). Control plants produced higher root number (17.93 ± 5.57) but with shorter roots. Tuber formation was observed only in control and IBA treatments, The results indicate that aeroponics combined with IBA enhances rooting efficiency, root growth and tuber development, and can be used for rapid propagation and conservation of this threatened species.