In the C. Personata groundnut A. hypogaea
(susceptible cv. GG-11) interaction, NADPH oxidase
enzyme activity was induced 24 hours after the inoculation
which increased cons-tantly. On TLC profiling, one unique
band was only found in disease state of groundnut having λ
max at 305nmt in UV spectral scanning. GC–MS study
showed to be a Hexade-canoic acid methyl ester with molecular formula C18H36O2. The IR spectrum (KBr, ν, cm-1):
2852-2926 (aliphatic-C-H str.), 1603 (-C=O str.), 1120 (C-O
ester str.) showed absorptions at 1603 and 1120 cm-1
indicating the presence of carbonyl and ester functional
group respectively. The 1H NMR and The 13C NMR spectrum obtained were in agreement with present compound was
Hexa decanoic acid methyl ester also called palmitic acid
having molecular formula C18H36O2. Palmitic acid resulted
in increased production of the NADPH oxidase and
subsequent production of reactive oxygen species (ROS).
Simultaneous presence of palmitic acid in disease state and
induced activity of NADPH oxidase activity with enhanced
disease incidence in groundnut shows relationship between
palmitic acid and NADPH oxidase during pathogenecity.
Thus, palmitic acid mediated induction of NADPH oxidase
in groundnut might be the factor to focus to combat
pathogenesis of C. personata.