Neem oil is well known for its application in the field of agriculture, medicine, energy, toiletries, cosmetics and others. Investigations have shown that the oil content as well as qualitative characters of fatty oils of oil seed species is highly influenced by agro climatic and phytogeographic conditions to which they are exposed. In order to identify good provenances yielding better quality seeds, the seed oil of different provenances was examined for its physico—chemical studies. The present work deals with the studies regarding variability in physico—chemical constants such as refractive index, acid value, saponification value and percentage of unsaponifiable matter of Neem oil obtained from the seeds of different provenances.