Microbiology is an important field of life science. Students of U.G. as well as P.G. in life science come across the techniques in microbiology every now and then. They face difficulty in finding the proper techniques and protocols related to different microbes under a single headed book. The book covers all the techniques commonly and routinely used in the microbiology laboratory and has been conveniently divided into 14 chapters with an elaborated appendix consisting of 120 types of important microbiological media, indicators and commonly used reagents. The unique feature of this book is that it includes the elaborated study of fungi and actinomycetes. Besides it provides detailed information on staining and maintenance of cultures.
This is essential reading for all life science undergraduate and postgraduate students and researchers as well
1 : Techniques in Microscopy; Intro-duction; Types of microscopes; Use of immer-sion oil; Measurement of microorganisms 2: Techniques of sterilization: Intro-duction; Moist sterilization; Sterili-zation; Other sterilization methods 3: Culture Medium: Introduction; Types of media; Sterilization; Storage of media 4 : Control of Microorganisms: Introd- uction; Physical methods of control; Chemical methods of control 5: Growing Microorganisms: Introdu-ction; Culture techniques; Culture of anaer-obic microbes; Culture characteristics of microorganisms 6: Techniques of Isolation: Introduction; Isolation of fungi; Isolation of bacteria; Sub culturing of microorganisms; Precautions for the use of culture 7: Maintenance of Culture: General main-tenance; Lyophilization; Sordelli's method 8: Estimation of Micro-organisms: Introduction; The total count; The viable count 9: Techniques of Microbial Staining: Introduction; Types of stains; Simple staining; Differential staining; Structural staining 10: Techniques of cultivation of Bact-eria : Introduction; Culture conditions; Maintenance of culture; Methods for isolation of bacteria 11: Techinques of Cultivation of Fungi: Introduction; Media; Maintenance of culture; Isolation of fungi; Culture of fungi for morphological studies; Stains for microscopic observation of fungi; The microscopic characteristics of fungi in culture; The macroscopic characteristics of fungi in culture; Measuring the growth of fungi; Enzyme action of fungi; Antibacterial activity of fungi; Toxic action of fungi 12: Techniques of cultivation of Actino-mycetes: Introduction; Cultivation and observation of actinomycetes; Methods of staining; Isolation techniques 13: Study of Microbial growth: Introdu-ction; The lag phase; The logarithmic phase; The stationary phase; The phase of decline; The turbidimetric method 14: Effects of environmental factors: Introduction; Effect of temperature; Effect of osmotic pressure; Ionic effects; Effect of oxygen; Effect of aeration; Effect of desiccation