The applications of Microbiology, Biochemistry and Molecular
Biology have led to the accumulation of a huge body of knowledge and
phenomenal growth in procedures and methodologies. In recent years
many novel methods have been developed and the old methods have been
improved. Nevertheless the time tested classical techniques have been
retained. The comprehensive practical training for any student studying
biological sciences is must. Though many practical books are available in
the market but this is an unique combination of protocols that covers
maximum (about 80%) of the practicals of various Indian universities for
UG and PG courses in Bioscience, Biotechnology, Microbiology,
Biochemistry and Biochemical Engineering.
While preparing this laboratory manual the efforts have been made
into various aspects of laboratory practices for the beginners like; the do’s
and don’ts of working in any laboratory, concepts and terminologies used,
and how to prepare the solutions/reagents. The protocols given here have
been tested by authors during their long teaching experience. The book
has been divided into four sections, the first one is Introduction which is
subdivided into laboratory etiquette and safety, molecular, empirical and
formula weight, planning a solution of a particular molarity, accuracy and
calibration, buffers etc. Second part is about Instruments: Principle
and Precautions which elaborates various commonly used equipments
needed to perform different experiments. The third part of the book is
about Experiments which has all the traditional to latest experiments
with principle, requirements, procedure, results and observation and
precautions in three major areas of Bioscience and Biotechnology i.e.
Microbiology, Biochemistry and Molecular Biology. At the end a
rather comprehensive Appendix is given as section four.