In the present study, induction of physical and chemical mutagens was used to hand pick morpho-logical mutants in M2 and M3 generations. Not much evidence is accessible on the nutrient and biochemical composition of Phaseolus lunatus L. Hence in the current investigation, an attempt has been made to comprehend the nutritional and biochemical valuation of Phaseolus lunatus L. (lima bean) which is consumed by tribals. The biochemical analysis of leaf, seed and immature pod content of lima bean were used to estimate chlorophyll (a, b) and protein content. Maximum chlorophyll content was observed in dark green leaf mutant and minimum was documented in robust mutant. Total volume of chlorophyll in lima bean was highly significant and had positive correlation with Chl a and Chl b at 1% significance level. In current estimation of proteins, the pods, dry seeds and leaves displayed increase in total protein in many viable mutants of lima bean. As related to control protein in seeds increased as the dose/concentration of gamma rays, ethyl methanesulphonate (EMS) and combination of both mutagens increased. Leaf protein content in M3 generation mutants showed 4 to 6%. Highest total protein was seen in dark green leaf and lowermost was in robust mutant. Compared to control there was positive shift in mean value of the total leaf protein. Total protein content for immature pods raised to 3.9 to 5.9%. Early flowering mutants had maximum and tall mutants had minimum protein value. Dark green leaf mutant (27.8) had maximum and late flowering mutant (30.0%) had minimum total protein content in them.