Section I: GENERAL TECHNIQUES IN PATHOLOGY
1. Lab Safety Guidelines
2. SI Units and Conventional Units used in
Hospital Lab
3. Various Instruments and Glassware used in
Lab
4. Microscope: Use and Care of Microscope, Dark
Ground Microscope
and Fluorescent Microscope
5. Maintenance of Records and Filing of
Slides
Section II: HEMATOLOGY
6. Haemopoiesis and Stem cells
7. Blood in General: Normal Constituents of
Blood, Collection of Blood
samples, Sequence of Filling of Tube and Anticoagulants
8. Hemoglobin Estimation
9. PCV (Packed cell volume) or Hematocrit
10. ESR : Erythrocyte Sedimentation Rate
11. Absolute Eosinophil Count
12. Anemia: IDA, Megaloblastic, Pernicious and
Thalassemia
13. Calculation of Red Cell Indices
14. Preparation and Staining of PBF for
Morphology of RBC and WBC, TLC, TRBC, TPC (Total
Platelet Count)
15. Reticulocyte Count
16. Investigation of Megaloblastic Anemia, B12
and Folate Assay, Schilling Test
17. Investigation of Iron Deficiency Anemia,
Estimation of Serum Iron and Iron Binding Capacity
18. Test for Autoimmune Hemolytic Anemia
19. Hemoglobin Electrophoresis
20. Sickling Tests
21. Osmotic Fragility Test
22. Determination of HbF and HbA2
23. Investigation of G6-PD
24. Plasma Haptoglobin and Hemosiderin in
Urine
25. Measurement of Abnormal Hb Pigment
26. Normal Hemostasis
27. Bleeding Time (BT) and Clotting Time
(CT)
28. Prothrombin Time (PT) and Activated Partial Thromboplastin Time (APTT)
29. Assay of Clotting Factors: Euglobulin Test,
Clot Lysis Test, FDP and Test for
Fibrinolytic Activity
30. Platelet Function Tests
31. Normal Fibrinolytic System
32. Demonstration of LE Cells
33. Bone Marrow Aspiration
34. Preparation of Bone Marrow Aspiration Smear
and Staining
35. Bone Marrow Biopsy Study
36. Cytochemistry of Bone Marrow in D/D of
Leukemia
37. Quality Management System
Section III: BLOOD BANKING
38. Blood Banking
39. Blood Components
Section IV: CYTOPATHOLOGY
40. Introduction to Clinical Pathology or Laboratory Medicine
41. Collection, Transport, Preservation and
Processing of Various
Clinical Specimens
42. Collection and Preservation of Urine
43. Physical Examination of Urine
44. Chemical Examination of Urine
45. Microscopic Examination of Urine
46. Examination of CSF
47. Examination of Pleural Fluid
48. Examination of Ascitic Fluid
49. Examination of Synovial Fluid
50. Sputum Examination
51. Examination of Faeces or Stool
52. Infertility
Section V: CYTOLOGY
53. Introduction to Cytology
54. Cytology: Normal Cell Structure, Function and
Cytologic Criteria
of Malignancy
55. Types of Specimen, Method of Collection and
Preparation of Cell
Block
56. Different Fixatives and Methods of
Fixation
57. Papanicoloau’s Stain, Principle, Preparation
and Staining Techniques
58. May Grunwald Giemsa Stain
59. Shorr’s Stain
60. Aceto Orcin Stain
61. Female Genital Tract: Anatomy, Histology,
Physiology and Normal Cytology
62. Technique of Collection of Specimens of
Cervical Scrapping for Cytology
63. Female Genital Tract: Hormonal Cytology and
Cytological Indices
64. Female Genital Tract: Cervical Cytology
Screening for Malignant and Non-Malignant Conditions
65. Cytology of Endometrium
66. Cytology of Ovarian Cancers
67. Respiratory Tract: Anatomy, Histology and
Physiology
68. Collection of Samples from Respiratory Tract
and Preparation of Smear and Staining
69. Respiratory Tract: Cytology of Normal,
Malignant and NonMalignant Conditions
70. GIT: Anatomy, Histology, Physiology of
Gastrointestinal Tract
71. Collection of Samples from Gastrointestinal
Tract and Preparation of Smears and Staining
72. Gastrointestinal Tract: Cytology of Normal,
Malignant and Non-Malignant conditions
73. Urinary Tract: Anatomy, Histology and
Physiology
74. Collection of Sample from Urinary Tract and
Preparation of Smears and Staining
75. Urinary Tract Cytology: Normal, Malignant and
Non Malignant
Conditions
76. CSF Cytology, Normal, Inflammatory,
Nonmalignant and Malignant Conditions
77. Ascetic fluid Cytology - Normal, Non
Malignant and Malignant Conditions
78. Pleural Fluid Cytology: Normal, Non Malignant
and Malignant Conditions
79. Synovial Fluid Cytology: Normal, Non
malignant and Malignant Conditions
80. Breast: Anatomy, Histology and
Physiology
81. FNAC of Breast
82. Cytology of Breast: Nonmalignant and Malignant Conditions
83. Thyroid Anatomy, Histology and
Physiology
84. FNAC of Thyroid
85. Cytology of Thyroid: Non Malignant and Malignant
Conditions
86. Salivary Glands Anatomy, Histology and
Physiology
87. FNAC of Salivary Glands
88. Cytology of Salivary Glands: Nonmalignant and
Malignant Conditions
89. Lymphnode: Anatomy, Histology and Physiology
90. FNAC of Lymphnode
91. Cytology of Lymph Node: Nonmalignant and Malignant conditions
92. Automation in Cytology: Flow Cytometry
93. Automation in Cytology: Image Analysis
94. Automation in Cytology: Principles and
Evaluation in Automation
Section VI: HISTOPATHOLGY
95. Introduction to Histopathology
96. Receiving of Specimen in Laboratory
97. Grossing Technique
98. Fixatives
99. Decalcification of Tissue
100. Tissue Processing
101. Section Cutting (Microtomy)
102. Staining of Tissue
103. Mounting Techniques
104. Automated Tissue Processor
105. Microtome and Ultramicrotome
106. Knives and Knife Sharpeners
107. Freezing Microtome
108. Cryostat (Refrigerated Microtome)
109. Routine Paraffin Section Cutting
110. Frozen Section
111. Automatic Slide Stainer
112. Special Stains for Carbohydrate
113. Special Stains for Connective Tissue
114. Special Stains for Nerves Tissue
115. Special Stains for Bone Tissue
116. Special Stains for Collagen Fibers and
Elastin Fibers
117. Special Stains for Lipid
118. Special Stains for Fungi
119. Special Stains for Parasite
120. Special Stains for Different Pigments and
Deposits in Tissues
121.
Museum Technology
122.
Neoplasia and Carcinogenesis
Section VII: BIOMEDICAL WASTE MANAGEMENT
123.
Biomedical Waste Management
Section VIII: ADVANCES IN PATHOLOGY
124.
Electron Microscope
125.
Microphotography and Its Applications
126.
International Classification of Diseases (ICDs) and Coding
127.
Applications of Computers in Pathology
128.
Cytochemistry - Part I (in Hematology)
129.
Cytochemistry Part II
130.
Equipment for Tissue Culture Studies- Laminar Flow
131.
CO2 Incubation for Tissue Culture Studies
132.
Inverted Microscope for Tissue Culture Studies
133.
Derivation of Culture from Tissue Enzymatic Digestion
134.
Plating in Tissue Culture
135.
Subculturing and Derivation in Tissue Culture
136.
Characterization of Cells Lines: Determination of Biochemical
Markers
137.
Characterization of Cells Lines: Chromosomal and DNA Content of Cells,
Immunological Properties
138.
Preservation of Immortalized Cell Lines: Storage in Glycerol or
Dimethyl Sulphoxide in Liquid Nitrogen
139.
Cytogenetics: Introduction, Terminology, Classification and Nomenclature of
Human Chromosome
140.
Methods of Karyotyping Analysis: Culture of Bone Marrow Cells,
Peripheral Blood, Lymphocytes, Solid Tumors and Skin Fibroblast
141.
Characterization of Chromosome Banding Technique
142.
Sex Chromatin (Barr Body)
143.
Identification of Chromosomes in Neoplasia and Oncogenes
144.
Immunohistochemistry (IHC): Basic Concept, Monoclonal Antibodies and their
Preparation
145.
Fluorescence Reactions in IHC
146.
IHC (Immuno-Histochemistry) and Diagnosis of Malignancy by Tumor Marker
147.
HPLC (High Performance Liquid Chromatography)
148.
Next Generation Sequencing and Liquid Biopsy
Bibliography
Question Papers of Previous Examination
Subject Index
