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Detection and Diagnosis of Plant Diseases

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Detection and Diagnosis of Plant Diseases

A.K. Meena , S.L. Godara & P.N. Meena

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Blurb

The present book “Detection and Diagnosis of Plant Diseases” deals with actual practical trends in modern Plant Pathology. It furnishes protocol on recent advances in bio-chemicals, biotechnological methods and aims to cover many important aspects such as Plant Pathology, Microbiology, Agricultural Microbiology, Biochemistry and Molecular biology. This book is designed to need the practical requirement of graduate and post-graduate students studying Plant Pathology, Microbiology, Biotechnology and Biochemistry courses by providing a readymade solution to the most of common experiments prescribed by any Indian University. Beside the latest technological development given in the book can be of interest to researchers and scientists. Most attention is given to the principal and theory behind various protocols that are expanding in details to aid understanding. It contains fifteen chapters emphasized on good laboratory practices in introduction to Plant Pathology as well as Microbiological equipments, isolation of plant pathogens from plants samples and soil samples, evaluation of fungicide toxicity by various methods, plant diseases diagnosis; field and laboratory diagnosis and important serological and molecular techniques, important biochemical methods, preparation of buffer solutions and at last is various important information related to agriculture graduate and post graduate students.

Instruments used in the study of plant diseases/pathogens

Methods to Prove Koch’s postulates

Preparation of culture media for the growth and isolation of fungi and bacteria

Isolation of plant pathogens

Staining of fungal pathogens

Staining of bacterial pathogens

Isolation and purification of plant pathogenic fungi from rhizosphere soil

Isolation and purification of plant pathogenic bacteria from rhizosphere soil

Plant disease diagnosis: the complete process of field observations, sample collection, sample packaging and laboratory diagnosis

Identification of Plant Parasitic Nematode-Heterodera

Identification of Plant Parasitic Nematode-Meloidogyne

Identification of Plant Parasitic Nematode-Anguina

Extraction of Nematode From Soil by Sieving and Decantation Method

Sampling and Extraction of Nematodes From Diseased Plant Materials

Preparation of Nematode Mounting

Preservation of disease samples by dry and wet methods

Evaluation of fungicide toxicity by food poisoning method against pathogens

Important serological and molecular techniques and instruments in Plant Pathology

Random Amplified Polymorphic DNA ( RAPD), PCR: Polymerase Chain Reaction

Enzyme Linked Immune Sorbent Assay (ELISA)

Dot- Immune Binding Assay (DIBA)

Immunosorbent Electron Microscopy (ISEM)

Isolation of fungal genomic DNA

Restriction Fragment Length Polymorphism (RFLP)

Amplified Fragment Length Polymorphism

Centrifuge, Spectrophotometer, PH meter, Microscopy (Phase Contrast Microscopy), Chromatography, Camera Lucida

Preparation of buffer and reagents

Methods of biochemical analyses

Estimation of Chlorophyll

Estimation of Proline

Estimation of Phenol

Estimation of Protein

Estimation of Total sugar (Reducing and Non-Reducing)

Estimation of Polyphenol Oxidase (PPO)

Estimation of Catalase (CAT) enzyme

Estimation of Phenyl-alanine Ammonia Lyase (PAL)

Estimation of Peroxidase (POD)

Estimation of Superoxide Dismutase (SOD)

General conversion tables and formulae

(Common conversions of nucleic acids and proteins)

Important plant pathological web sites 

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