The study of plant diversity within national parks and wildlife reserves is important for the effective habitat management of wildlife and their habitat. This paper describes the floral composition, local distribution and use of plants in Royal Shuklaphanta Wildlife Reserve in southwestern Nepal. The area comprises different habitat types such as forests, grasslands, floodplains, lakes and marshes,and is famous for a number of wildlife species. The study was conducted from September 1997 to December 1999. It included the collection and identification of flowering plants and interviews with the local people about their uses. Altogether 562 plant species belonging to 102 family and 375 genera were recorded. Seventy four species are newly recorded in western lowlands of Nepal and among them, 26 species are new additions to the flora of Nepal. Twenty seven species were found locally rare within the reserve. Local people were found to use 208 species for various purposes. Royal Shuklaphanta Wildlife Reserve is rich in plant diversity, but its management measures may be needed to maintain species richness within the reserve.