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Detection and Diagnosis of Plant Diseases

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Detection and Diagnosis of Plant Diseases

A.K. Meena , S.L. Godara & P.N. Meena

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The present book Detection and Diagnosis of Plant Diseases deals with actual practical trends in modern Plant Pathology. It furnishes protocol on recent advances in bio-chemicals, biotechnological methods and aims to cover many important aspects such as Plant Pathology, Microbiology, Agricultural Microbiology, Biochemistry and Molecular biology. This book is designed to need the practical requirement of graduate and post-graduate students studying Plant Pathology, Microbiology, Biotechnology and Biochemistry courses by providing a ready made solution to the most of common experiments prescribed by any Indian University. Beside the latest technological development given in the book can be of interest to researchers and scientists. Most attention is given to the principal and theory behind various protocols that are expanding in details to aid understanding. It contains fifteen chapters emphasized on good laboratory practices in introduction to Plant Pathology as well as Microbiological equipments, isolation of plant pathogens from plants samples and soil samples, evaluation of fungicide toxicity by various methods, plant diseases diagnosis; field and laboratory diagnosis and important serological and molecular techniques, important biochemical methods, preparation of buffer solutions and at last is various important information related to agriculture graduate and post graduate students.

1. Instruments used in the study of plant diseases/pathogens

2. Methods to Prove Koch’s postulates

3. Preparation of culture media for the growth and isolation of fungi and bacteria

4. Isolation of plant pathogens

5. Staining of fungal pathogens

6. Staining of bacterial pathogens

7. Isolation and purification of plant pathogenic fungi from rhizosphere soil

8. Isolation and purification of plant pathogenic bacteria from rhizosphere soil

9. Plant disease diagnosis: the complete process of field observations, sample collection, sample packaging and laboratory diagnosis

10. Identification of Plant Parasitic Nematode-Heterodera

11. Identification of Plant Parasitic Nematode-Meloidogyne

12. Identification of Plant Parasitic Nematode-Anguina

13. Extraction of Nematode From Soil by Sieving and Decantation Method

14. Sampling and Extraction of Nematodes From Diseased Plant Materials

15. Preparation of Nematode Mounting

16. Preservation of disease samples by dry and wet methods

17. Evaluation of fungicide toxicity by food poisoning method against pathogens

18. Important serological and molecular techniques and instruments in Plant Pathology

            Random Amplified Polymorphic DNA ( RAPD), PCR: Polymerase Chain Reaction

            Enzyme Linked Immune Sorbent Assay (ELISA)

            Dot- Immune Binding Assay (DIBA)

            Immunosorbent Electron Microscopy (ISEM)

            Isolation of fungal genomic DNA

            Restriction Fragment Length Polymorphism (RFLP)

            Amplified Fragment Length Polymorphism

            Centrifuge, Spectrophotometer, PH meter, Microscopy (Phase Contrast Microscopy),
            Chromatography, Camera Lucida

19. Preparation of buffer and reagents

20. Methods of biochemical analyses

            Estimation of Chlorophyll

            Estimation of Proline

            Estimation of Phenol

            Estimation of Protein

            Estimation of Total sugar (Reducing and Non-Reducing)

            Estimation of Polyphenol Oxidase (PPO)

            Estimation of Catalase (CAT) enzyme

            Estimation of Phenyl-alanine Ammonia Lyase (PAL)

            Estimation of Peroxidase (POD)

            Estimation of Superoxide Dismutase (SOD)

21. General Information

            Conversion tables and formula

            Plant Pathology Related Web Sites

            Important Symptoms of Plant Diseases

            Terminology

            Suggested Readings

            Composition of Different Media Used for Fungus and Bacterial Growth

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