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Home Books Agriculture Entomology Handbook of Practical Nematology

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Nematology is the discipline of science that deals with the study of nematodes or roundworms. Current requirements of practical nematological work and unavailability of expertise and studies in this field, indicates a dire need to encourage the likes of this book on the neglected groups of organisms. The authors, in the present book have attempted to cover the practical aspects of Nematology for U.G. and P.G. students, teachers and research workers. Rather than enlisting numerous methods adopted by various workers, emphasis is given to most commonly used techniques for every excercise. Additionally, to gain in depth knowledge on a particular topic, further readings have been suggested at the end of each exercise. Illustrations have also been given that will help the readers in identification of common nematode species and disease symptoms caused by them.

Exercise 1. Collection and preservation of soil and plant samples - Harish K. Bajaj
Collection of soil samples
Collection and preservation of plant parts

Exercise 2. Isolation of nematodes from soil and plant parts and their estimation - Harish K. Bajaj
   Isolation and collection of nematodes from soil
   Isolation and collection of active nematodes and cysts
   Isolation and collection of sessile, slow moving or moulting nematodes
   Isolation and collection of nematodes from Plant Parts
   Isolation and collection of migratory endoparasitic nematodes
   Isolation and collection of sedentary endo- and semiendoparasitic forms
   Counting nematode population in suspension

Exercise 3. Preparation of nematode slides for morphology and taxonomic studies - Harish K. Bajaj
   Preparation of temporary mounts
   Killing, fixing and preserving nematodes
   Preparation of permanent yoto mounts
   Preparation of perineal pattern, vulval cone and en face view

Exercise 4. Measurements of nematodes - Harish K. Bajaj
   Measurements with an ocular micrometer
   Measurements with camera lucida
   Measuring nematodes and their body parts

Exercise 5. Identification scheme for common genera of plant parasitic nematodes of India - Harish K. Bajaj

Exercise 6. Identification of common cyst and root-knot nematode species of India - Harish K. Bajaj
Identification of cyst nematodes
Identification of Meloidogyne Species

Exercise 7. Identification of host races of important nematode species of India - Harish K. Bajaj
   Identification of host races of Meloidogyne incognita, M. arenaria and M. javanica
   Identification of host races of Rotylenchulus reniformis
   Identification of host races of Heterodera cajani
   Identification of pathotypes of Heterodera avenae
   Identification of host races of Heterodera zeae
   Identification of host races of Globodera rostochiensis and G. pallida

Exercise 8. Life cycle studies of nematodes - Harish K. Bajaj
   Embryonic development (Hanging drop method)
   Post embryonic development
   Variations with other major nematode groups

Exercise 9. Histopathology of nematode infected roots - P.P.S. Baghel

Exercise 10. Pathogenicity trials for plant parasitic nematodes - K.R. Dabur

Exercise 11. Major nematode pests of important crops in India : Morphology, symptomatology and control - Harish K. Bajaj & R.S. Kanwar
   Anguina tritici
   Aphelenchoides species
   Ditylenchus angustus
   Heterodera and Globodera species
   Meloidogyne species
   Rotylenchulus reniformis
   Tylenchulus semipenetrans
Radopholus similis
   Hirschmanniella species
Pratylenchus species

Exercise 12. Crop loss assessment due to nematodes - R.S. Kanwar & Harish K. Bajaj
Estimation of avoidable losses in an infested
Estimation of losses in a geographical area

Exercise 13. In vitro screening of chemicals against nematodes - I.J. Paruthi
Mortality Test
Hatching Test

Exercise 14. Testing of chemicals against plant parasitic nematodes by different methods in pots and field - R.K. Jain
   Evaluation of chemicals by seed soaking method for the control of root-knot nematode
   Efficacy of chemicals used as seed dressing method for the control of root-knot nematode
   Evaluation of chemicals for the control of root knot nematode through bare root-dip method
   Evaluation of granular nematicides for the control of root-knot nematode as soil application / nursery bed treatment

Exercise 15. Testing phytotherapeutic substances for nematode control - R.S. Kanwar
Evaluation of plant extracts for their toxicity against root-knot nematode
Evaluation of efficacy of plant parts for root-knot nematode control

Exercise 16. Screening of germpalsm for resistance against important phytoparasitic nematodes - R.K. Jain
   Meloidogyne species on vegetables and pulse crops
   Rotylenchulus reniformis on vegetable and pulse crops
   Anguina tritici on wheat
   Heterodera avenae on wheat and barley
   Heterodera cajani on pulse crops
   Tylenchulus semipenetrans on citrus
   Radopholus similis on coconut
   Ditylenchus angustus on rice

Exercise 17. Field trials on biocontrol agents for the management of phytonematodes - R.K. Walia
Paecilomyces lilacinus
Pasteuria penetrans

Exercise 18. Crop rotation trials for management of plant parasitic nematodes - R.S. Kanwar

Exercise 19. In vitro culturing of plant parasitic and fungivorus nematodes - R. S. Kanwar
   In vitro culturing of plant parasitic nematodes
   Callus tissue technique
   Carrot disc technique
   Excised root technique
   In vitro culturing of fungal feeding nematodes

Exercise 20. Isolation and culturing of entomopathogenic nematodes - Harish K. Bajaj
Isolation and mass rearing of EPNs in vivo
Collection of adults of EPNs and studies on their life cycles